Dynamical investigation of macromolecular hybridization bioassays
نویسندگان
چکیده
A novel sensoric technique for the dynamical in situ investigation of a hybridization bio assay is presented, which utilizes a metal bead labeling method. Therein, hybridization results in an increased metal coverage on parts of a substrate, where it takes place. Our sensing principle relies on the measurement of the radio frequency impedance of the hybridization spots. We propose several examples for sensor devices. The hybridization of macromolecules like DNA or specific proteins for biochemical, medical or forensic purposes is usually observed applying fluorescence or radioactive markers. As the therefore required labeling processes are usually quite complicated, invasive, and also expensive, there is a growing need for detection of hybridization processes utilizing standardized, simple and cheap labeling. Moreover, standard hybridization assays usually only generate so calledènd point´results, i.e. the kinetics of the hybridization process cannot be studied. Apart from using fluorescent dyes or radioactive markers, the application of nano-scale gold-beads for labeling purposes has also been reported upon [1]. On the one hand, the gold-beads can be used for labeling the sample molecule, which binds specifically to its counterpart on a micro-array. On the other hand, a two part sandwich assay can be realized, which consists of a defined binding site for the sample molecule, a labeled head group in solution, and the sample molecule acting as a specific linker between the both [2,3].
منابع مشابه
Investigation of TEL/AML1 and BCR/ABL Fusion Genes in Patients Affected by Acute Lymphoblastic Leukaemia Using Interphase in situ Hybridization
متن کامل
Investigation of Dynamical Behavior (Transverse Vibration) and Instability Analysis of Carbon Nanotubes Conveying Nanofluid
This work focuses on the dynamical behavior of carbon nanotubes, including vibration, wave propagation and fluid-structure interaction. In the present research, transverse vibration of nano fluid conveying carbon nanotubes is investigated. To this end, based on the nonlocal and strain-inertia gradient continuum elasticity theories and by using rod and Euler-Bernoulli beam models, the system’s d...
متن کاملSecond Harmonic Generation Diagnostic of Layer by Layer Deposition from Disperse Red 1–Functionalized Maleic Anhydride Copolymer
Layer-by-layer (LBL) electrostatic assembly of poly-electrolytes is proving to be an increasingly rich and versatile technique for the formation of multilayered thin films with a wide range of electrical, magnetic, and optical properties. In the present work we synthesized a new nonlinear optical (NLO) maleic acid copolymer containing Disperse Red 1 moieties, built-up multilayer assemblies by a...
متن کاملBioassays Based on Molecular Nanomechanics
Recent experiments have shown that when specific biomolecular interactions are confined to one surface of a microcantilever beam, changes in intermolecular nanomechanical forces provide sufficient differential torque to bend the cantilever beam. This has been used to detect single base pair mismatches during DNA hybridization, as well as prostate specific antigen (PSA) at concentrations and con...
متن کاملScrambling reaction between polymers prepared by step-growth and chain-growth polymerizations: macromolecular cross-metathesis between 1,4-polybutadiene and olefin-containing polyester.
Controlled hybridization of olefin-containing polymers prepared by two different polymerization methods, step-growth polymerization and chain-growth polymerization, was accomplished by macromolecular olefin cross-metathesis.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2002